Difference between revisions of "Part:BBa K2356002"

 
 
Line 7: Line 7:
  
 
In this project, the specific tobacco isoform 14-3-3c is used and stripped of its last 18 C-terminal amino acids, called T14-3cΔC. This allows higher affinity towards the CT52 peptide, more specifically the YDI tail (see Figure 1), in the presence of small molecule fusicoccin.[2]
 
In this project, the specific tobacco isoform 14-3-3c is used and stripped of its last 18 C-terminal amino acids, called T14-3cΔC. This allows higher affinity towards the CT52 peptide, more specifically the YDI tail (see Figure 1), in the presence of small molecule fusicoccin.[2]
 +
 +
The protein mass is 60 kDa.
  
 
[1] Obsilova V, Kopecka M, Kosek D, Kacirova M, Kylarova S. Mechanisms of the 14-3-3 Protein Function : Regulation of Protein Function Through Conformational Modulation. 2014;63.
 
[1] Obsilova V, Kopecka M, Kosek D, Kacirova M, Kylarova S. Mechanisms of the 14-3-3 Protein Function : Regulation of Protein Function Through Conformational Modulation. 2014;63.

Latest revision as of 10:18, 27 October 2017


14-3-3 dimer

Proteins belonging to the 14-3-3 family are dimers, where each monomer consists out of nine anti-parallel α-helices. This causes the dimer to obtain a cup-like shape with two amphipathic binding grooves. The structure forms a rigid scaffold that is capable of anchoring proteins. 14-3-3 proteins are involved in multiple cellular processes and are mostly known to bind phosphorylated peptide motifs, especially those containing phosphoserine and phosphothreonine sequences. Most regions are conserved among different 14-3-3 isoforms, but the C-terminus appears to show more variability and is important in binding different target proteins.[1]


In this project, the specific tobacco isoform 14-3-3c is used and stripped of its last 18 C-terminal amino acids, called T14-3cΔC. This allows higher affinity towards the CT52 peptide, more specifically the YDI tail (see Figure 1), in the presence of small molecule fusicoccin.[2]

The protein mass is 60 kDa.

[1] Obsilova V, Kopecka M, Kosek D, Kacirova M, Kylarova S. Mechanisms of the 14-3-3 Protein Function : Regulation of Protein Function Through Conformational Modulation. 2014;63. [2] Ottmann C, Marco S, Jaspert N, et al. Article Structure of a 14-3-3 Coordinated Hexamer of the Plant Plasma Membrane H + -ATPase by Combining X-Ray Crystallography and Electron Cryomicroscopy. 2007:427-440. doi:10.1016/j.molcel.2006.12.017.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1328
    Illegal EcoRI site found at 1483
    Illegal PstI site found at 543
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1328
    Illegal EcoRI site found at 1483
    Illegal PstI site found at 543
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1328
    Illegal EcoRI site found at 1483
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1328
    Illegal EcoRI site found at 1483
    Illegal PstI site found at 543
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1328
    Illegal EcoRI site found at 1483
    Illegal PstI site found at 543
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1031