Difference between revisions of "Part:BBa K2309022:Experience"
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The picture shows the dot blotting result of produced LL-37 which induced by IPTG in BL21 strain. The left one shows the peptide LL-37 produced and right is non-induced BL21 by IPTG. | The picture shows the dot blotting result of produced LL-37 which induced by IPTG in BL21 strain. The left one shows the peptide LL-37 produced and right is non-induced BL21 by IPTG. | ||
| − | For LL-37 ability assay, we test Minimal Inhibitory concentration(MIC) of Staphylococcus aureus, the result as | + | For LL-37 ability assay, we test Minimal Inhibitory concentration(MIC) of Staphylococcus aureus, the result as follows: |
https://static.igem.org/mediawiki/parts/5/5c/LL-37_MIC_XJTLU-CHINA.jpeg | https://static.igem.org/mediawiki/parts/5/5c/LL-37_MIC_XJTLU-CHINA.jpeg | ||
Revision as of 10:13, 27 October 2017
Applications of BBa_K2309022
This year we use dot blot method to evaluate part K2309022 work.
The picture shows the dot blotting result of produced LL-37 which induced by IPTG in BL21 strain. The left one shows the peptide LL-37 produced and right is non-induced BL21 by IPTG.
For LL-37 ability assay, we test Minimal Inhibitory concentration(MIC) of Staphylococcus aureus, the result as follows:
As picture shows that the concentration of 5-10ug/ml, the LL-37 has highly activity.
We also test the ability of LL-37 attact biofilm of Staphylococcus aureus, the result as follows:
The result shows that the value of 595nm ABS is dicrease while the increase of LL-37 concentration. It seems that LL-37 peptide inhibite Staphylococcus aureus to form the biofilm.
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