Difference between revisions of "Part:BBa K2302006"
(→‎RESULT)
Line 14: Line 14:
 
To further determine whether the recombinant CALB is functional, we made soft agar containing glyceryl tributyrate on culture plates containing growing bacteria. The result showed that Follower D could secrete degrade glyceryl tributyrate as indicated by transparent ring shaped as “NEFU”, suggesting its production of functional lipase.  
 
To further determine whether the recombinant CALB is functional, we made soft agar containing glyceryl tributyrate on culture plates containing growing bacteria. The result showed that Follower D could secrete degrade glyceryl tributyrate as indicated by transparent ring shaped as “NEFU”, suggesting its production of functional lipase.  
  
−
https://static.igem.org/mediawiki/2017/7/78/NEFU2O17--RESULTS--FIG13.jpg
 
 
https://static.igem.org/mediawiki/parts/f/fa/NEFU-China_2017%284%29.png
 
https://static.igem.org/mediawiki/parts/f/fa/NEFU-China_2017%284%29.png
 +
https://parts.igem.org/File:NEFU-China_2017_(3).jpg
  
 +
https://static.igem.org/mediawiki/parts/6/61/NEFU-China_2017_%283%29.jpg
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2302006 parameters</partinfo>
 
<partinfo>BBa_K2302006 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Revision as of 10:07, 27 October 2017


CALB,an enzyme that can hydrolyze fat to fatty acids.

this sequence encodes a functional protein that can hydrolyze fat to fatty acids. The protein is one of the most widely used lipases due to its excellent properties, such as high stereoselectivity and stability.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 949
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 670
    Illegal NgoMIV site found at 780
    Illegal AgeI site found at 472
    Illegal AgeI site found at 730
  • 1000
    COMPATIBLE WITH RFC[1000]

RESULT

To further determine whether the recombinant CALB is functional, we made soft agar containing glyceryl tributyrate on culture plates containing growing bacteria. The result showed that Follower D could secrete degrade glyceryl tributyrate as indicated by transparent ring shaped as “NEFU”, suggesting its production of functional lipase.

NEFU-China_2017%284%29.png https://parts.igem.org/File:NEFU-China_2017_(3).jpg

NEFU-China_2017_%283%29.jpg