Difference between revisions of "Part:BBa K2449028"

Latest revision as of 07:58, 27 October 2017

RelB with RBS and double terminator

A toxin-antitoxin system is a system composed of two gene products, of which one specifies a cell toxin and the other an antitoxin, which neutralizes the toxic effect caused by the toxin. In E. coli K-12 the cytotoxin RelE and antitoxin RelB comprise such a system [1]. The part BBa_K2449029 encodes the toxin RelE.

RelB neutralize the toxic effect of RelE through interaction between the two proteins.

[1] Gotfredsen M, Gerdes K. The Escherichia coli relBE genes belong to a new toxin-antitoxin gene family. Molecular microbiology.

Sequence and Features
RelB cloned with RBS site and a double terminator.
RelB was placed under control of the pBAD/AraC promoter in composite part, BBa_K2449031.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 121
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2449028 short</partinfo>
-A toxin-antitoxin system is a system composed of two gene products, of which one specifies a cell toxin and the other an antitoxin, which neutralizes the toxic effect caused by the toxin. In <i>E. coli</i> K-12 the cytotoxin RelE and antitoxin RelB comprise such a system [1].
+A toxin-antitoxin system is a system composed of two gene products, of which one specifies a cell toxin and the other an antitoxin, which neutralizes the toxic effect caused by the toxin. In <i>E. coli</i> K-12 the cytotoxin RelE and antitoxin RelB comprise such a system [1]. The part [https://parts.igem.org/Part:BBa_K2449029 BBa_K2449029] encodes the toxin RelE.
 RelB neutralize the toxic effect of RelE through interaction between the two proteins.
 [1] Gotfredsen M, Gerdes K. The Escherichia coli relBE genes belong to a new toxin-antitoxin gene family. Molecular microbiology.
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