Difference between revisions of "Part:BBa K2449028"
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<partinfo>BBa_K2449028 short</partinfo>
 
<partinfo>BBa_K2449028 short</partinfo>
  
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A toxin-antitoxin system is a system composed of two gene products, of which one specifies a cell toxin and the other an antitoxin, which neutralizes the toxic effect caused by the toxin. In <i>E. coli</i> K-12 the cytotoxin RelE and antitoxin RelB comprise such a system [1].
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A toxin-antitoxin system is a system composed of two gene products, of which one specifies a cell toxin and the other an antitoxin, which neutralizes the toxic effect caused by the toxin. In <i>E. coli</i> K-12 the cytotoxin RelE and antitoxin RelB comprise such a system [1].  
  
 
RelB neutralize the toxic effect of RelE through interaction between the two proteins.
 
RelB neutralize the toxic effect of RelE through interaction between the two proteins.
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RelB was placed under control of the pBAD/AraC promoter in composite part, [https://parts.igem.org/Part:BBa_K2449031 BBa_K2449031].
 
  
 
[1] Gotfredsen M, Gerdes K. The Escherichia coli relBE genes belong to a new toxin-antitoxin gene family. Molecular microbiology.
 
[1] Gotfredsen M, Gerdes K. The Escherichia coli relBE genes belong to a new toxin-antitoxin gene family. Molecular microbiology.
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<span class='h3bb'><b>Sequence and Features</b><br>RelB cloned with RBS site and a double terminator.</span>
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<span class='h3bb'><b>Sequence and Features</b><br>RelB cloned with RBS site and a double terminator.<br>RelB was placed under control of the pBAD/AraC promoter in composite part, [https://parts.igem.org/Part:BBa_K2449031 BBa_K2449031].</span>
 
<partinfo>BBa_K2449028 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2449028 SequenceAndFeatures</partinfo>
  

Revision as of 07:55, 27 October 2017


RelB with RBS and double terminator

A toxin-antitoxin system is a system composed of two gene products, of which one specifies a cell toxin and the other an antitoxin, which neutralizes the toxic effect caused by the toxin. In E. coli K-12 the cytotoxin RelE and antitoxin RelB comprise such a system [1].

RelB neutralize the toxic effect of RelE through interaction between the two proteins.

[1] Gotfredsen M, Gerdes K. The Escherichia coli relBE genes belong to a new toxin-antitoxin gene family. Molecular microbiology.

Sequence and Features
RelB cloned with RBS site and a double terminator.
RelB was placed under control of the pBAD/AraC promoter in composite part, BBa_K2449031.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 121
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]