Difference between revisions of "Part:BBa K2449028"

Revision as of 07:53, 27 October 2017

RelB with RBS and double terminator

A toxin-antitoxin system is a system composed of two gene products, of which one specifies a cell toxin and the other an antitoxin, which neutralizes the toxic effect caused by the toxin. In E. coli K-12 the cytotoxin RelE and antitoxin RelB comprise such a system [1].

RelB neutralize the toxic effect of RelE through interaction between the two proteins.

RelB was placed under control of the pBAD/AraC promoter in composite part, BBa_K2449031.

[1] Gotfredsen M, Gerdes K. The Escherichia coli relBE genes belong to a new toxin-antitoxin gene family. Molecular microbiology.

Sequence and Features
RelB cloned with RBS site and a double terminator.