Difference between revisions of "Part:BBa K2381005:Design"

(→‎References)
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2381005 short</partinfo>
 
<partinfo>BBa_K2381005 short</partinfo>
 +
A fusion protein of nMagHigh and Cod2, which linked by GS linker.
  
 
<partinfo>BBa_K2381005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2381005 SequenceAndFeatures</partinfo>

Revision as of 07:45, 27 October 2017


cod + nMag A fusion protein of nMagHigh and Cod2, which linked by GS linker.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1740
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 403


Design Notes

In order to prevent the fusion protein has steric hindrance, We use a 12aa GS linker to link the light-induced dimerization protein with a part of dCas9, which split by a conventional strategy. According to the paper, this conventional strategy is effective by rapamycin induced.

Source

The light-induced protein nMagHigh is made from the VVD protein family derived from the fungus Neurospora crassa. The cod2 is made from the dCas9 Protein derived from the Streptococcus pyogenes.

References

Nihongaki, Y., et al. (2015). "Photoactivatable CRISPR-Cas9 for optogenetic genome editing." Nat Biotechnol 33(7): 755-760.