Difference between revisions of "Part:BBa K2449029"

 
Line 3: Line 3:
 
<partinfo>BBa_K2449029 short</partinfo>
 
<partinfo>BBa_K2449029 short</partinfo>
  
−
RelE toxin.  
+
A toxin-antitoxin system is a system composed of two gene products, of which one specifies a cell toxin and the other an antitoxin, which neutralizes the toxic effect caused by the toxin. In <i>E. coli</i> K-12 the cytotoxin RelE and antitoxin RelB comprise such a system [1].
 +
 
 +
Expression of the cytotoxin RelE inhibits translation in the cells, due to its ability to cleave mRNA found in the A-site of the ribosome [2]. An indigenous factor for expression of RelE in <i>E. coli</i> is conditioned by amino acid starvation [2].
 +
 
 +
[1] Gotfredsen M, Gerdes K. The Escherichia coli relBE genes belong to a new toxin-antitoxin gene family.<br>[2] Pedersen K, Zavialov AV, Pavlov MY, Elf J, Gerdes K, Ehrenberg M. The bacterial toxin RelE displays codon-specific cleavage of mRNAs in the ribosomal A site.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 9: Line 13:
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
<span class='h3bb'><b>Sequence and Features</b><br>
 +
RBS and double terminator are added the RelE gene.
 +
</span>
 
<partinfo>BBa_K2449029 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2449029 SequenceAndFeatures</partinfo>
−
 
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 07:44, 27 October 2017


RelE with RBS and double terminator

A toxin-antitoxin system is a system composed of two gene products, of which one specifies a cell toxin and the other an antitoxin, which neutralizes the toxic effect caused by the toxin. In E. coli K-12 the cytotoxin RelE and antitoxin RelB comprise such a system [1].

Expression of the cytotoxin RelE inhibits translation in the cells, due to its ability to cleave mRNA found in the A-site of the ribosome [2]. An indigenous factor for expression of RelE in E. coli is conditioned by amino acid starvation [2].

[1] Gotfredsen M, Gerdes K. The Escherichia coli relBE genes belong to a new toxin-antitoxin gene family.
[2] Pedersen K, Zavialov AV, Pavlov MY, Elf J, Gerdes K, Ehrenberg M. The bacterial toxin RelE displays codon-specific cleavage of mRNAs in the ribosomal A site.

Sequence and Features
RBS and double terminator are added the RelE gene.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]