Difference between revisions of "Part:BBa K2381003:Design"
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===Source=== | ===Source=== | ||
| − | + | The light-induced protein pMagFast is made from the VVD protein family derived from the fungus Neurospora crassa. The cod is made from the dCas9 Protein derived from the Streptococcus pyogenes. | |
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===References=== | ===References=== | ||
Nihongaki, Y., et al. (2015). "Photoactivatable CRISPR-Cas9 for optogenetic genome editing." Nat Biotechnol 33(7): 755-760. | Nihongaki, Y., et al. (2015). "Photoactivatable CRISPR-Cas9 for optogenetic genome editing." Nat Biotechnol 33(7): 755-760. | ||
Revision as of 07:25, 27 October 2017
cod1 + pMag
A fusion protein of pMagFast and Cod1, which linked by GS linker.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1099
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2256
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In order to prevent the fusion protein has steric hindrance, We use a 12aa GS linker to link the light-induced dimerization protein with a part of dCas9, which split by a conventional strategy. According to the paper, this conventional strategy is effective by rapamycin induced.
Source
The light-induced protein pMagFast is made from the VVD protein family derived from the fungus Neurospora crassa. The cod is made from the dCas9 Protein derived from the Streptococcus pyogenes.
References
Nihongaki, Y., et al. (2015). "Photoactivatable CRISPR-Cas9 for optogenetic genome editing." Nat Biotechnol 33(7): 755-760.