Difference between revisions of "Part:BBa K2260000:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Insert considerations here (e.g. codon optimization in E. coli, removal of restriction sites, etc.)
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<p>
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The phaCBA operon was codon optimized for use in <i>E. coli</i>  using the codon optimization table. Furthermore, four restrictions sites present in the original operon obtained from <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1149051">BBa_K1149051</a> were removed, including any new restriction sites introduced from codon optimization.
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===Source===
 
===Source===

Revision as of 06:55, 27 October 2017


PhaCBA operon w/ Histidine tags


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The phaCBA operon was codon optimized for use in E. coli using the codon optimization table. Furthermore, four restrictions sites present in the original operon obtained from <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1149051">BBa_K1149051</a> were removed, including any new restriction sites introduced from codon optimization.

Source

Insert bacterial species' genome(s) here.

References