Difference between revisions of "Part:BBa K2275007"
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<partinfo>BBa_K2275007 short</partinfo>
 
<partinfo>BBa_K2275007 short</partinfo>
  
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This composite part encodes nitrite reductase cluster (nirB and nirD) which derived from E. coli K-12 MG1655 strain. In order to achieve our goal of regulation, we choose lacI regulated promoter R0010 and B0034 RBS for high expression level.  
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This composite part is composed of R0010, B0034 and K2275001 which encodes nitrite reductase derived from E. coli K-12 MG1655 strain.  
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Then we used Griess reagent assay to test the activity of NiR
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Then we used Griess reagent assay to test the activity of NiR. The principle and mechanism of Griess reaction are shown below. When the broth contains nitrite, by adding the Griess reactant which consists of naphthylethylenediamine dihydrochloride, sulphanilamide,  phosphoric acid can form a diazonium salt with nitrite. The diazonium salt will have absorbance at wavelength 545 nm. By the reaction, we can further calculate the nitrate conversion.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 04:42, 27 October 2017


PlacI-B0034-NiR cluster

This composite part is composed of R0010, B0034 and K2275001 which encodes nitrite reductase derived from E. coli K-12 MG1655 strain. Then we used Griess reagent assay to test the activity of NiR. The principle and mechanism of Griess reaction are shown below. When the broth contains nitrite, by adding the Griess reactant which consists of naphthylethylenediamine dihydrochloride, sulphanilamide, phosphoric acid can form a diazonium salt with nitrite. The diazonium salt will have absorbance at wavelength 545 nm. By the reaction, we can further calculate the nitrate conversion.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 286
    Illegal BglII site found at 1287
    Illegal BglII site found at 2070
    Illegal BamHI site found at 567
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2093
    Illegal AgeI site found at 551
    Illegal AgeI site found at 947
    Illegal AgeI site found at 2105
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 401