Difference between revisions of "Part:BBa K2381013"
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<p>this part can response to blue light (470nm), rapidly bind to the corresponding parts, <partinfo>BBa_K2381014</partinfo>, due to eletrostatic interaction and dimerize. Its cofactor for light sensing is flavin adenine dinucleotide (FAD). After dimerization the split luciferase will complement to recover its abillity to generate fluorescenece. | <p>this part can response to blue light (470nm), rapidly bind to the corresponding parts, <partinfo>BBa_K2381014</partinfo>, due to eletrostatic interaction and dimerize. Its cofactor for light sensing is flavin adenine dinucleotide (FAD). After dimerization the split luciferase will complement to recover its abillity to generate fluorescenece. | ||
</p> | </p> | ||
+ | [[File:HZAU_2017_MagLuc.png|400px|thumb|left|Fig.1 Prove the function of pMag & nMag. n=1]] | ||
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===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 04:00, 27 October 2017
nMag-LucC
this part can response to blue light (470nm), rapidly bind to the corresponding parts, BBa_K2381014, due to eletrostatic interaction and dimerize. Its cofactor for light sensing is flavin adenine dinucleotide (FAD). After dimerization the split luciferase will complement to recover its abillity to generate fluorescenece.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 762
Illegal AgeI site found at 901 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 403