Difference between revisions of "Part:BBa K2440021"

 
Line 3: Line 3:
 
<partinfo>BBa_K2440021 short</partinfo>
 
<partinfo>BBa_K2440021 short</partinfo>
  
−
Null.
+
It is the target sequence of miR2118l, a modularized DNA part from a set of chemically synthetic oligo DNA library.
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
MiRNA locker assembled by using this modularized DNA part was able to bind miR2118l in an Ago2 dependent manner, that is, knockdown of miR2118l was achieved by transfecting cells with miRNA locker.
 +
 +
===Sequence and Features===
  
 
<!-- -->
 
<!-- -->
Line 17: Line 20:
 
<partinfo>BBa_K2440021 parameters</partinfo>
 
<partinfo>BBa_K2440021 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
==Experimental Validation==
 +
 +
 +
This part is sequenced as correct after construction.

Revision as of 03:15, 27 October 2017


miR2118l target sequence

It is the target sequence of miR2118l, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR2118l in an Ago2 dependent manner, that is, knockdown of miR2118l was achieved by transfecting cells with miRNA locker.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.