Difference between revisions of "Part:BBa K2440016"

 
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<partinfo>BBa_K2440016 short</partinfo>
 
<partinfo>BBa_K2440016 short</partinfo>
  
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Null.
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It is the target sequence of miR-511, a modularized DNA part from a set of chemically synthetic oligo DNA library.
  
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===Usage and Biology===
 
===Usage and Biology===
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MiRNA locker assembled by using this modularized DNA part was able to bind miR-511 in an Ago2 dependent manner, that is, knockdown of miR-511 was achieved by transfecting cells with miRNA locker.
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===Sequence and Features===
  
 
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<partinfo>BBa_K2440016 parameters</partinfo>
 
<partinfo>BBa_K2440016 parameters</partinfo>
 
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==Experimental Validation==
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This part is sequenced as correct after construction.

Revision as of 03:04, 27 October 2017


miR-511 target sequence

It is the target sequence of miR-511, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-511 in an Ago2 dependent manner, that is, knockdown of miR-511 was achieved by transfecting cells with miRNA locker.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 4
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 4
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.