Difference between revisions of "Part:BBa K2440012"
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It is the target sequence of miR-4470, a modularized DNA part from a set of chemically synthetic oligo DNA library. | It is the target sequence of miR-4470, a modularized DNA part from a set of chemically synthetic oligo DNA library. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
MiRNA locker assembled by using this modularized DNA part was able to bind miR-4470 in an Ago2 dependent manner, that is, knockdown of miR-4470 was achieved by transfecting cells with miRNA locker. | MiRNA locker assembled by using this modularized DNA part was able to bind miR-4470 in an Ago2 dependent manner, that is, knockdown of miR-4470 was achieved by transfecting cells with miRNA locker. | ||
| + | ===Sequence and Features=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
| − | <partinfo> | + | <partinfo>BBa_K2440011 SequenceAndFeatures</partinfo> |
Revision as of 02:47, 27 October 2017
miR-4470 target sequence
It is the target sequence of miR-4470, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR-4470 in an Ago2 dependent manner, that is, knockdown of miR-4470 was achieved by transfecting cells with miRNA locker.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.