Difference between revisions of "Part:BBa K2440012"
| Line 3: | Line 3: | ||
<partinfo>BBa_K2440012 short</partinfo> | <partinfo>BBa_K2440012 short</partinfo> | ||
| − | + | It is the target sequence of miR-4470, a modularized DNA part from a set of chemically synthetic oligo DNA library. | |
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | |||
| + | MiRNA locker assembled by using this modularized DNA part was able to bind miR-4470 in an Ago2 dependent manner, that is, knockdown of miR-4470 was achieved by transfecting cells with miRNA locker. | ||
<!-- --> | <!-- --> | ||
| Line 17: | Line 20: | ||
<partinfo>BBa_K2440012 parameters</partinfo> | <partinfo>BBa_K2440012 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
| + | |||
| + | ==Experimental Validation== | ||
| + | |||
| + | |||
| + | This part is sequenced as correct after construction. | ||
Revision as of 02:45, 27 October 2017
miR-4470 target sequence
It is the target sequence of miR-4470, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.