Difference between revisions of "Part:BBa K2381013"
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<partinfo>BBa_K2381013 short</partinfo>
 
<partinfo>BBa_K2381013 short</partinfo>
  
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<p>It is a fusion protein fused N fragment of split luciferase  with light-induced dimerization domain. It is used to prove that our light-induced dimerization domain is working.
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<p>this part can response to blue light (470nm), rapidly bind to the corresponding parts due to eletrostatic interaction and dimerize. Its cofactor for light sensing is flavin adenine dinucleotide (FAD). After dimerization the split luciferase will complement to recover its abillity to generate fluorescenece.  
 
</p>
 
</p>
 
<!-- Add more about the biology of this part here
 
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Revision as of 01:41, 27 October 2017


nMag-LucC

this part can response to blue light (470nm), rapidly bind to the corresponding parts due to eletrostatic interaction and dimerize. Its cofactor for light sensing is flavin adenine dinucleotide (FAD). After dimerization the split luciferase will complement to recover its abillity to generate fluorescenece.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 762
    Illegal AgeI site found at 901
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 403