Difference between revisions of "Part:BBa K2428000"

Revision as of 23:21, 26 October 2017

Mutagenized psB1C3 to create blunt-end restriction sites for cloning not within the prefix/suffix

This part, psB1C3mut, is a circularized form of the plasmid backbone psB1C3. Specifically, the UChicago team cut the linearized psB1C3 sent from iGEM with XbaI and SpeI, then ligated the DNA back together to create a circularized psB1C3 plasmid. QuickChange PCR was performed to create blunt-end restriction sites (such as HpaI) for cloning not within the prefix/suffix region of the original psB1C3 iGEM plasmid. Contains chloramphenicol resistance.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1012
Illegal XhoI site found at 1904
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2428000 short</partinfo>
-This part, psB1C3mut, is a circularized form of the plasmid backbone psB1C3. Specifically, the UChicago team cut the linearized psB1C3 sent from iGEM with XbaI and SpeI, then ligated the DNA back together to create a circularized psB1C3 plasmid. QuickChange PCR was performed to create blunt-end restriction sites (such as HpaI) for cloning not within the prefix/suffix region of the original psB1C3 iGEM plasmid.
+This part, psB1C3mut, is a circularized form of the plasmid backbone psB1C3. Specifically, the UChicago team cut the linearized psB1C3 sent from iGEM with XbaI and SpeI, then ligated the DNA back together to create a circularized psB1C3 plasmid. QuickChange PCR was performed to create blunt-end restriction sites (such as HpaI) for cloning not within the prefix/suffix region of the original psB1C3 iGEM plasmid. Contains chloramphenicol resistance.
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