Difference between revisions of "Part:BBa K2292005"

 
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This construction is used for lactate quantization
 
This construction is used for lactate quantization
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We made use of the property of LldR operon. The LldR is transcription factor. Without lactate, it will bind on O1、O2 operator site, form a DNA loop, blocking the transcription. As lactate present, lactate will bind on LldR and open the loop, allowing the transcription to go on and produce GFP. GFP intensity is then detected and deduct lactate level.
+
We made use of the property of LldR operon. The LldR is transcription factor. Without lactate, it will bind on O1&O2 operator site, form a DNA loop, blocking the transcription. As lactate present, lactate will bind on LldR and open the loop, allowing the transcription to go on and produce GFP. GFP intensity is then detected and deduct lactate level.
 
This construction is cloned into E.coli.  
 
This construction is cloned into E.coli.  
 +
 +
https://i.imgur.com/dLFTci6.jpg
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 +
O1 and O2 binding is added on two sides of promoter(J23118)
 +
 +
Sequence:
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O1: AATTGGCCCTACCAATT
 +
O2: AATTGGCAGTGCCACTT
  
  

Revision as of 20:18, 26 October 2017


Lactate detection

Our project aims to detect the risk of caries through three parameters: Competence stimulating peptide, CSP concentration, lactate level, pH value.

This construction is used for lactate quantization We made use of the property of LldR operon. The LldR is transcription factor. Without lactate, it will bind on O1&O2 operator site, form a DNA loop, blocking the transcription. As lactate present, lactate will bind on LldR and open the loop, allowing the transcription to go on and produce GFP. GFP intensity is then detected and deduct lactate level. This construction is cloned into E.coli.

dLFTci6.jpg

O1 and O2 binding is added on two sides of promoter(J23118)

Sequence:

O1: AATTGGCCCTACCAATT O2: AATTGGCAGTGCCACTT


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 993
    Illegal NheI site found at 1016
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 628
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1691