Difference between revisions of "Part:BBa K2292003"
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This is a composite used for producing comD, comE, two essential protein for the CSP two components signal transduction system. | This is a composite used for producing comD, comE, two essential protein for the CSP two components signal transduction system. | ||
Originated from Streptococcus mutans. ComD is a kinase and membrane-associated receptor. After comD binded to the quorum sensing molecule CSP, the comD-CSP complex will phosphorylate comE. Then, the phosphorylated comE can turn on the transcription of downstream gene. | Originated from Streptococcus mutans. ComD is a kinase and membrane-associated receptor. After comD binded to the quorum sensing molecule CSP, the comD-CSP complex will phosphorylate comE. Then, the phosphorylated comE can turn on the transcription of downstream gene. | ||
| + | FLAG-TAG is added after comE and c-Myc is added after comD | ||
https://i.imgur.com/AjavUGq.jpg | https://i.imgur.com/AjavUGq.jpg | ||
Revision as of 19:37, 26 October 2017
comDE two components signal transduction system
This is a composite used for producing comD, comE, two essential protein for the CSP two components signal transduction system. Originated from Streptococcus mutans. ComD is a kinase and membrane-associated receptor. After comD binded to the quorum sensing molecule CSP, the comD-CSP complex will phosphorylate comE. Then, the phosphorylated comE can turn on the transcription of downstream gene. FLAG-TAG is added after comE and c-Myc is added after comD
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 268
Illegal XhoI site found at 123
Illegal XhoI site found at 153 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]