Difference between revisions of "Part:BBa K2520012"

Revision as of 19:18, 26 October 2017

EF1a-Mono display:pro insulin-hGH

This device is a complete system for displaying proteins on cells' membrane. The Igk leader (BBa_K2520024) is a short signal peptide that prompts the translocation of a protein to the cellular membrane. PDGFR (BBa_K2520026) is a trans-membrane domain that anchors all the components located between the igk leader and the PDGFR itself to the membrane. HA (BBa_K2520030) and Myc (BBa_K2520031) are tags that bind to specific antibodies and provide an indirect method for verifying the display of proteins on the membrane. The protein that we chose to express on the membrane is an epitope that is known target of diabetes (BBa_K2520027) and is component related to our specific project. The EF1a promoter (BBa_K2520023) is a constitutive promoter in mammalian cells and hGH (BBa_K404108) serves as a terminator. EF1a promoter is very useful in cells where other promoters, such as CMV, are underactive or silenced (such as stem cells).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 643
Illegal XhoI site found at 1042
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 777
Illegal AgeI site found at 151
Illegal AgeI site found at 1315
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2762

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2520012 short</partinfo>
-EF1a promoter-mono pro insulin-hGH
+This device is a complete system for displaying proteins on cells' membrane. The Igk leader (<partinfo>K2520024</partinfo>) is a short signal peptide that prompts the translocation of a protein to the cellular membrane. PDGFR (<partinfo>K2520026</partinfo>) is a trans-membrane domain that anchors all the components located between the igk leader and the PDGFR itself to the membrane. HA (<partinfo>K2520030</partinfo>) and Myc (<partinfo>K2520031</partinfo>) are tags that bind to specific antibodies and provide an indirect method for verifying the display of proteins on the membrane. The protein that we chose to express on the membrane is an epitope that is known target of diabetes (<partinfo>K2520027</partinfo>) and is component related to our specific project.
+The EF1a promoter (<partinfo>K2520023</partinfo>) is a constitutive promoter in mammalian cells and hGH (<partinfo>K404108</partinfo>) serves as a terminator. EF1a promoter is very useful in cells where other promoters, such as CMV, are underactive or silenced (such as stem cells).
 <!-- Add more about the biology of this part here