Difference between revisions of "Part:BBa K2382007"

Line 5: Line 5:
 
Since bacteria can’t produce the whole antibody, we decide to use scFv to
 
Since bacteria can’t produce the whole antibody, we decide to use scFv to
 
replace it. It contains two polypeptide chains linked by a linker. And we add a start coden (ATG)
 
replace it. It contains two polypeptide chains linked by a linker. And we add a start coden (ATG)
 +
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 18:04, 26 October 2017


Anti-aflatoxin scFv (with start coden)

Since bacteria can’t produce the whole antibody, we decide to use scFv to replace it. It contains two polypeptide chains linked by a linker. And we add a start coden (ATG)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 342
    Illegal SapI.rc site found at 255