Difference between revisions of "Part:BBa K2516000"

 
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__NOTOC__
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<partinfo>BBa_K2516000 short</partinfo>
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Chromate reductase (ChrR) is an oxidoreductase enzyme that is able to transfer 3 electrons towards Cr6+ anions, which results in reduction directly into Cr3+ ions. This reduction avoids production of side products such as Cr5+ and Cr4+, which are quite toxic to the cell. Both NADH and NADPH are equally utilized as electron donors. Other substrates that can be reduced: quinones (primary biological role), potassium ferricyanide, 2,6-dichloroindophenol, V5+, Mo6+, methylene blue, cytochrome c and U6+.
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<b>Reaction of electron transfer is the following:</b>
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2 NAD(P)H + Cr6+ + O2 = 2 NAD(P)+ + Cr3+ + superoxide
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<b>Flavin mononucleotide (FMN)</b> serves as a cofactor molecule.
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This enzyme lacks any post-translational modifications.
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<b>Optimal Conditions:</b>
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Optimum pH (for Cr6+) = 5
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Optimum temperature (for Cr6+) = 35 degrees Celsius
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<b>Enzyme kinetics:</b>
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Vmax (for Cr6+) = 5 µmol/min/mg at optimal conditions
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Km (for Cr6+) = 200 uM at optimal conditions
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2516000 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K2516000 parameters</partinfo>
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Revision as of 17:59, 26 October 2017


Chromate reductase (codon-optimized for C. reinhardtii)

Chromate reductase (ChrR) is an oxidoreductase enzyme that is able to transfer 3 electrons towards Cr6+ anions, which results in reduction directly into Cr3+ ions. This reduction avoids production of side products such as Cr5+ and Cr4+, which are quite toxic to the cell. Both NADH and NADPH are equally utilized as electron donors. Other substrates that can be reduced: quinones (primary biological role), potassium ferricyanide, 2,6-dichloroindophenol, V5+, Mo6+, methylene blue, cytochrome c and U6+.


Reaction of electron transfer is the following:

2 NAD(P)H + Cr6+ + O2 = 2 NAD(P)+ + Cr3+ + superoxide


Flavin mononucleotide (FMN) serves as a cofactor molecule.

This enzyme lacks any post-translational modifications.


Optimal Conditions:

Optimum pH (for Cr6+) = 5

Optimum temperature (for Cr6+) = 35 degrees Celsius


Enzyme kinetics:

Vmax (for Cr6+) = 5 µmol/min/mg at optimal conditions

Km (for Cr6+) = 200 uM at optimal conditions

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 319
  • 1000
    COMPATIBLE WITH RFC[1000]