Difference between revisions of "Part:BBa K2520001"
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A scFv is a fusion protein of the variable regions of an antibody (VH and VL) connected by a short linker peptide.  This fusion protein retains the specificity of the original antibody despite the removal of the constant regions.  
 
A scFv is a fusion protein of the variable regions of an antibody (VH and VL) connected by a short linker peptide.  This fusion protein retains the specificity of the original antibody despite the removal of the constant regions.  
  
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[[File:scfv_small.png|300px|thumb|center|Figure 1: Structure of the Tri-Display. Each display component consist of Igk leader, tag, epitope and PDGFR]]
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[[File:scfv_small.png|300px|thumb|center|Figure 1: Structure of scFv. VH=variable heavy, VL=variable light]]
  
 
This fusion protein can be secreted or displayed on the cellular membrane.
 
This fusion protein can be secreted or displayed on the cellular membrane.

Revision as of 17:56, 26 October 2017


Single Chain Fragment Variable (scFv) of rat anti-murine IgM antibody

Single Chain Fragment Variable (scFv) A scFv is a fusion protein of the variable regions of an antibody (VH and VL) connected by a short linker peptide. This fusion protein retains the specificity of the original antibody despite the removal of the constant regions.

Figure 1: Structure of scFv. VH=variable heavy, VL=variable light

This fusion protein can be secreted or displayed on the cellular membrane.


References

Lee, Timothy K., et al. "Production of engineered IgM-binding single-chain antibodies in Escherichia coli." Journal of Industrial Microbiology & Biotechnology 14.5 (1995): 371-376. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]