Difference between revisions of "Part:BBa K2520001"
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A scFv is a fusion protein of the variable regions of an antibody (VH and VL) connected by a short linker peptide.  This fusion protein retains the specificity of the original antibody despite the removal of the constant regions.  
 
A scFv is a fusion protein of the variable regions of an antibody (VH and VL) connected by a short linker peptide.  This fusion protein retains the specificity of the original antibody despite the removal of the constant regions.  
  
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[[File:scfv_small.png|800px|thumb|center|Figure 1: Structure of the Tri-Display. Each display component consist of Igk leader, tag, epitope and PDGFR]]
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[[File:scfv_small.png|400px|thumb|center|Figure 1: Structure of the Tri-Display. Each display component consist of Igk leader, tag, epitope and PDGFR]]
  
 
This fusion protein can be secreted or displayed on the cellular membrane.
 
This fusion protein can be secreted or displayed on the cellular membrane.

Revision as of 17:49, 26 October 2017


Single Chain Fragment Variable (scFv) of rat anti-murine IgM antibody

Single Chain Fragment Variable (scFv) A scFv is a fusion protein of the variable regions of an antibody (VH and VL) connected by a short linker peptide. This fusion protein retains the specificity of the original antibody despite the removal of the constant regions.

Figure 1: Structure of the Tri-Display. Each display component consist of Igk leader, tag, epitope and PDGFR

This fusion protein can be secreted or displayed on the cellular membrane.


References

Lee, Timothy K., et al. "Production of engineered IgM-binding single-chain antibodies in Escherichia coli." Journal of Industrial Microbiology & Biotechnology 14.5 (1995): 371-376. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]