Difference between revisions of "Part:BBa K2275007"
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<partinfo>BBa_K2275007 short</partinfo>
 
<partinfo>BBa_K2275007 short</partinfo>
  
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This composite part encodes nitrite reductase cluster (nirB and nirD) which derived from E. coli K-12 MG1655 strain. To achieve our goal of regulation, we choose lacI regulated promoter R0010 and B0034 RBS for high expression level .  
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This composite part encodes nitrite reductase cluster (nirB and nirD) which derived from E. coli K-12 MG1655 strain. In order to achieve our goal of regulation, we choose lacI regulated promoter R0010 and B0034 RBS for high expression level.  
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Then we used Griess reagent assay to test the activity of NiR
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:19, 26 October 2017


PlacI-B0034-NiR cluster

This composite part encodes nitrite reductase cluster (nirB and nirD) which derived from E. coli K-12 MG1655 strain. In order to achieve our goal of regulation, we choose lacI regulated promoter R0010 and B0034 RBS for high expression level. Then we used Griess reagent assay to test the activity of NiR

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 286
    Illegal BglII site found at 1287
    Illegal BglII site found at 2070
    Illegal BamHI site found at 567
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2093
    Illegal AgeI site found at 551
    Illegal AgeI site found at 947
    Illegal AgeI site found at 2105
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 401