Difference between revisions of "Part:BBa I742105"

Latest revision as of 12:47, 23 October 2007

Sam5 gene with ribosome binding site

This part includes the sam5 gene of the bacterium Saccharothrix espanaensis DSM 4429 along with its native ribosome binding site. The coding sequence alone, with no ribosome binding site, as also been submitted as BBa_I742142. Sam5 encodes a 4-coumarate 3-hydroxylase. The substrate is p-coumaric acid, which is converted to caffeic acid. This gene is naturally involved in caffeic acid biosynthesis (Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis' J. Bacteriol. 188 (7), 2666-2673 (2006)) and is part of the artifical vanillin biosynthesis pathway devised by the Edinburgh iGEM2007 team.

Please note that this version of the gene contains a PstI site near the 3' end of the coding sequence. This will be removed by site directed mutagenesis in due course, at which time this entry will be edited to reflect the availability of the revised part. The present version, including the PstI site, was prepared so as to test activity prior to undertaking the mutagenesis. It can only be inserted downstream of another biobrick (as a EcoRI-SpeI insert) or have another biobrick inserted downstream of it.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1436
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 1436
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1436
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1436
Illegal NgoMIV site found at 37
Illegal NgoMIV site found at 45
Illegal NgoMIV site found at 795
Illegal NgoMIV site found at 968
Illegal NgoMIV site found at 1370
Illegal NgoMIV site found at 1374
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 569

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 __NOTOC__
 <partinfo>BBa_I742105 short</partinfo>
-Sam5 encodes a 4-coumarate 3-hydroxylase.  The enzymes substrate is p-coumaric acid, which is converted to caffeic acid.  This gene is part of the vanillin biosynthesis pathway.
+This part includes the sam5 gene of the bacterium ''Saccharothrix espanaensis'' DSM 4429 along with its native ribosome binding site. The coding sequence alone, with no ribosome binding site, as also been submitted as BBa_I742142. Sam5 encodes a 4-coumarate 3-hydroxylase.  The substrate is p-coumaric acid, which is converted to caffeic acid.  This gene is naturally involved in caffeic acid biosynthesis (Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete ''Saccharothrix espanaensis''' J. Bacteriol. 188 (7), 2666-2673 (2006)) and is part of the artifical vanillin biosynthesis pathway devised by the Edinburgh iGEM2007 team.
+Please note that this version of the gene contains a PstI site near the 3' end of the coding sequence. This will be removed by site directed mutagenesis in due course, at which time this entry will be edited to reflect the availability of the revised part. The present version, including the PstI site, was prepared so as to test activity prior to undertaking the mutagenesis. It can only be inserted downstream of another biobrick (as a EcoRI-SpeI insert) or have another biobrick inserted downstream of it.
 <!-- Add more about the biology of this part here