Difference between revisions of "Part:BBa K2407306"
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__NOTOC__
 
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<partinfo>BBa_K2407306 short</partinfo>
 
<partinfo>BBa_K2407306 short</partinfo>
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==Introduction==
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This composite workes for inserting the vox sites and Ura3 gene between them can be replaced by any structural gene. Initially, Before the vika-vox system works, the β-carotene does not express. After mating, the component between two vox sites will be deleted by vika enzyme and the β-carotene gene will synthesize β-carotene. Yeasts turns to orange.
  
 
Initially, the composite regulates the expression of ura3. When the vika-vox system begin to work, the crtE gene with the remaining composite of β-carotene gene will synthesize β-carotene.
 
Initially, the composite regulates the expression of ura3. When the vika-vox system begin to work, the crtE gene with the remaining composite of β-carotene gene will synthesize β-carotene.

Revision as of 17:16, 26 October 2017


TEF Promoter + vox + Ura3 + Ura3 terminator + vox + β-carotene

Introduction

This composite workes for inserting the vox sites and Ura3 gene between them can be replaced by any structural gene. Initially, Before the vika-vox system works, the β-carotene does not express. After mating, the component between two vox sites will be deleted by vika enzyme and the β-carotene gene will synthesize β-carotene. Yeasts turns to orange.

Initially, the composite regulates the expression of ura3. When the vika-vox system begin to work, the crtE gene with the remaining composite of β-carotene gene will synthesize β-carotene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2145
    Illegal BglII site found at 3832
    Illegal XhoI site found at 1844
    Illegal XhoI site found at 1976
    Illegal XhoI site found at 2795
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2012
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1125
    Illegal BsaI site found at 4736
    Illegal BsaI site found at 7091
    Illegal BsaI.rc site found at 176
    Illegal BsaI.rc site found at 1572
    Illegal BsaI.rc site found at 7422
    Illegal SapI site found at 4136
    Illegal SapI.rc site found at 972