Difference between revisions of "Part:BBa K2407307"
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<partinfo>BBa_K2407307 short</partinfo>
 
<partinfo>BBa_K2407307 short</partinfo>
  
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Initially, the composite regulates the expression of Red Fluorescence Protein. When the vika-vox system begin to work, the crtE gene with the remaining composite of β-carotene gene will synthesize β-carotene.
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==Introduction==
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We use this composite to compare the phenomenon before and after mating. Initially, the Red Fluorescence Protein expresses. When the vika-vox system begin to work, the component between two vox sites will be deleted and the β-carotene gene will synthesize β-carotene. Yeasts turns from red to orange.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:05, 26 October 2017


TDH3 Promoter + vox + Ura + Ura3 terminator + vox + β-carotene

Introduction

We use this composite to compare the phenomenon before and after mating. Initially, the Red Fluorescence Protein expresses. When the vika-vox system begin to work, the component between two vox sites will be deleted and the β-carotene gene will synthesize β-carotene. Yeasts turns from red to orange.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2410
    Illegal BglII site found at 4097
    Illegal XhoI site found at 2109
    Illegal XhoI site found at 2241
    Illegal XhoI site found at 3060
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2277
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1390
    Illegal BsaI site found at 5001
    Illegal BsaI site found at 7356
    Illegal BsaI.rc site found at 1837
    Illegal BsaI.rc site found at 7687
    Illegal SapI site found at 4401
    Illegal SapI.rc site found at 1237