Difference between revisions of "Part:BBa K2382010"

Revision as of 17:00, 26 October 2017

Anti-aflatoxin scFv fusion protein construction DNA sequence

By ligating the constitutive promoter (BBa_J23101), strong ribosome binding site (BBa_B0034) and scFv-EAAAK- RFP-Histag, we were able to detect aflatoxin by the purified protein expressing in E. coli. Moreover, we designed a restriction site, BamHI, between scFv and EAAAK, so future iGEM teams could take advantage of this composite part to fuse their scFv with RFP as indicator, and make their own test strip!

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1342
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 403
Illegal SapI.rc site found at 316

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2382010 short</partinfo>
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+By ligating the constitutive promoter (BBa_J23101), strong ribosome
+binding site (BBa_B0034) and scFv-EAAAK- RFP-Histag, we were able to
+detect aflatoxin by the purified protein expressing in E. coli. Moreover, we
+designed a restriction site, BamHI, between scFv and EAAAK, so future iGEM
+teams could take advantage of this composite part to fuse their scFv with
+RFP as indicator, and make their own test strip!
 <!-- Add more about the biology of this part here