Difference between revisions of "Part:BBa K2275007"

 
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<partinfo>BBa_K2275007 short</partinfo>
 
<partinfo>BBa_K2275007 short</partinfo>
  
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NiR
+
This composite part encodes nitrite reductase cluster (nirB and nirD) which derived from E. coli K-12 MG1655 strain. To achieve our goal of regulation, we choose lacI regulated promoter R0010 and B0034 RBS for high expression level .
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:50, 26 October 2017


PlacI-B0034-NiR cluster

This composite part encodes nitrite reductase cluster (nirB and nirD) which derived from E. coli K-12 MG1655 strain. To achieve our goal of regulation, we choose lacI regulated promoter R0010 and B0034 RBS for high expression level .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 286
    Illegal BglII site found at 1287
    Illegal BglII site found at 2070
    Illegal BamHI site found at 567
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2093
    Illegal AgeI site found at 551
    Illegal AgeI site found at 947
    Illegal AgeI site found at 2105
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 401