Difference between revisions of "Part:BBa K2328055"

 
 
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__NOTOC__
 
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<partinfo>BBa_K2328055 short</partinfo>
 
<partinfo>BBa_K2328055 short</partinfo>
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nimomiya
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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<partinfo>BBa_K2328055 parameters</partinfo>
 
<partinfo>BBa_K2328055 parameters</partinfo>
 
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===Usage and Biology===
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Acma and smURFP are joined together, and the fragment is inserted into pMG36e to construct a new plasmid. When the plasmid is expressed, smURFP is anchored to the cell surface by Acma, thus expressing the shape. Linker II and Linker.a are used to separate Acma from other core parts .
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===Reference===
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[1] Li X, et al. Functional cell surface display of endo-beta-1, 3-1, 4-glucanase in Lactococcus lactis using N-acetylmuraminidase as the anchoring motif.Sheng Wu Gong Cheng Xue Bao. 2009.

Latest revision as of 16:33, 26 October 2017


AcmA + Linker II + Linker.a

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 324
    Illegal PstI site found at 437
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 324
    Illegal NheI site found at 606
    Illegal PstI site found at 437
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 324
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 324
    Illegal PstI site found at 437
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 324
    Illegal PstI site found at 437
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Acma and smURFP are joined together, and the fragment is inserted into pMG36e to construct a new plasmid. When the plasmid is expressed, smURFP is anchored to the cell surface by Acma, thus expressing the shape. Linker II and Linker.a are used to separate Acma from other core parts .

Reference

[1] Li X, et al. Functional cell surface display of endo-beta-1, 3-1, 4-glucanase in Lactococcus lactis using N-acetylmuraminidase as the anchoring motif.Sheng Wu Gong Cheng Xue Bao. 2009.