Difference between revisions of "Part:BBa K2520009"

 
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<partinfo>BBa_K2520009 short</partinfo>
 
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CMV-tTA-hGH
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Tetracycline-controlled transactivator protein (tTA) is composed of the Tet repressor DNA binding protein (TetR) from Escherichia coli fused to the strong transactivating domain of VP16 from Herpes simplex virus, regulates expression of a target gene that is under transcriptional control of a tetracycline-responsive promoter element (TRE).
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In the absence of Dox, tTA binds to the TRE promoter and activates transcription of the target gene. In the presence of Dox, tTA cannot bind to the TRE, and expression from the target gene remains inactive.
  
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We created a ready-to-use plasmid for tTA expression in mammalian cells under CMV promoter, by adding hGH poly A- a terminator for mammalian cells.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2520009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2520009 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
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<partinfo>BBa_K2520009 parameters</partinfo>
 
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Revision as of 16:09, 26 October 2017


CMV-tTA-hGH

Tetracycline-controlled transactivator protein (tTA) is composed of the Tet repressor DNA binding protein (TetR) from Escherichia coli fused to the strong transactivating domain of VP16 from Herpes simplex virus, regulates expression of a target gene that is under transcriptional control of a tetracycline-responsive promoter element (TRE). In the absence of Dox, tTA binds to the TRE promoter and activates transcription of the target gene. In the presence of Dox, tTA cannot bind to the TRE, and expression from the target gene remains inactive.

We created a ready-to-use plasmid for tTA expression in mammalian cells under CMV promoter, by adding hGH poly A- a terminator for mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1245
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1766