Difference between revisions of "Part:BBa K1491017"
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2017 SSTi-SZGD Team has improved the this part,The improvement was made by inserting supernova gene into a light-regulated expression system, lightoff (BBa_K2244009). | 2017 SSTi-SZGD Team has improved the this part,The improvement was made by inserting supernova gene into a light-regulated expression system, lightoff (BBa_K2244009). | ||
Revision as of 16:05, 26 October 2017
Codon Optimized Supernova
Supernova is the monomeric version of KillerRed (BBa_K1184000). It has been codon optimized for E. coli.
Of the three superoxide generators, Supernova had the biggest decrease in viability following photobleaching when compared to its control. It also had the largest decrease in fluorescence following photobleaching. Fluorescence was measured at (ex/em = 585nm/610nm).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 50
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
improvement
2017 SSTi-SZGD Team has improved the this part,The improvement was made by inserting supernova gene into a light-regulated expression system, lightoff (BBa_K2244009).