Difference between revisions of "Part:BBa K2323000"
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==Test==
 
===Functional Parameters===
 
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===Test2===
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Revision as of 15:51, 26 October 2017


Lwa Cas13a + Tphi terminator

Cas13a is a CRISPR/Cas effector that specifically binds and digests RNA based on its crRNA sequence. After digestion of this target sequence, Cas13a undergoes conformation change and presents collateral activity, i.e. it indiscriminately digests RNA molecules. The coding sequence is followed by Tphi terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1286
    Illegal BglII site found at 2966
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2365