Difference between revisions of "Part:BBa K2323000"
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2323000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2323000 SequenceAndFeatures</partinfo> | ||
+ | <partinfo>Benchling plasmid map and sequence: https://benchling.com/s/seq-IA6dwUFOSs7Tu0prM4VE</partinfo> | ||
Revision as of 15:39, 26 October 2017
Lwa Cas13a + Tphi terminator
Cas13a is a CRISPR/Cas effector that specifically binds and digests RNA based on its crRNA sequence. After digestion of this target sequence, Cas13a undergoes conformation change and presents collateral activity, i.e. it indiscriminately digests RNA molecules.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1286
Illegal BglII site found at 2966 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2365
No part name specified with partinfo tag.