Difference between revisions of "Part:BBa K2429019:Design"

(→‎Design Notes)
(→‎Source)
 
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===Source===
 
===Source===
  
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The human beta globin introns came from the HEK cell genome. The sequences for YFP and BFP were obtained through BLAST, and the part comes from the genome of a jellyfish.
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The human beta globin introns came from the HEK cell genome. YFP and BFP comes from the genome of a jellyfish.
  
 
===References===
 
===References===

Latest revision as of 14:59, 26 October 2017


3 Exon eYFP Reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2282
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2282
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2282
    Illegal BamHI site found at 1
    Illegal BamHI site found at 1412
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2282
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2282
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This basic part entry vector is flanked by L1 and L2 sites, which are used to denote a gene. The second HBG intron has a point mutation at bp 654 to match the intron used in a paper that used the HBG intron as part of their reporter.

Source

The human beta globin introns came from the HEK cell genome. YFP and BFP comes from the genome of a jellyfish.

References