Difference between revisions of "Part:BBa K2429016:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Ensure that the intron can be efficient spliced out of the construct.
+
This basic part entry vector is flanked by L1 and L2 sites, which are used to denote a gene.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 14:55, 26 October 2017


mKate-FF4 Reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 540
    Illegal EcoRI site found at 1157
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 540
    Illegal EcoRI site found at 1157
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 540
    Illegal EcoRI site found at 1157
    Illegal BamHI site found at 1
    Illegal XhoI site found at 437
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 540
    Illegal EcoRI site found at 1157
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 540
    Illegal EcoRI site found at 1157
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1062
    Illegal SapI.rc site found at 55


Design Notes

This basic part entry vector is flanked by L1 and L2 sites, which are used to denote a gene.

Source

N/A

References