Difference between revisions of "Part:BBa K2398555"

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Riboswitches, in general, are used for the binding of specific molecules to release an intrinsic RBS that leads to translation of the downstream gene of interest.   
 
Riboswitches, in general, are used for the binding of specific molecules to release an intrinsic RBS that leads to translation of the downstream gene of interest.   
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===Characterization===
  
  

Revision as of 14:19, 26 October 2017


Organosilicon binding Riboswitch

This in silico designed riboswitch by the MAWS software provided by the iGEM Team Heidelberg 2015 is made for the binding of Organosilicons. To be precise, ethyl 2-(dimethyl(phenyl)silyl)propanoate was used as the substrate. It can be used to 2.5-fold activate your downstream gene of interest as it is shown by our experiments. This part was ordered from IDT as oligo and oligo annealed, resulting in sticky ends to be golden gate assembled in pSB1C3 containing NLuc (Promega) as a reporter system. As riboswitches are only specific for one substrate this part is only validated for the use of the appropriate substrate.

Usage and Biology

Riboswitches, in general, are used for the binding of specific molecules to release an intrinsic RBS that leads to translation of the downstream gene of interest.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]