Difference between revisions of "Part:BBa K2398555"

Revision as of 14:11, 26 October 2017

Organosilicon binding Riboswitch

This in silico designed riboswitch by the MAWS software provided by the iGEM Team Heidelberg 2015 is made for the binding of Organosilicons. To be precise, ethyl 2-(dimethyl(phenyl)silyl)propanoate was used as the substrate. It can be used to 2.5-fold activate your downstream gene of interest as it is shown by our experiments. This part was ordered from IDT as oligo and oligo annealed, resulting in sticky ends to be golden gate assembled in pSB1C3 containing NLuc (Promega) as a reporter system. As riboswitches are only specific for one substrate this part is only validated for the use of the appropriate substrate.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2398555 short</partinfo>
-This <i>in silico</i> designed riboswitch by the MAWS software provided by the iGEM Team Heidelberg 2015 is made for the binding of Organosilicons. To be precise, ethyl 2-(dimethyl(phenyl)silyl)propanoate was used as the substrate.
+This <i>in silico</i> designed riboswitch by the MAWS software provided by the iGEM Team Heidelberg 2015 is made for the binding of Organosilicons. To be precise, ethyl 2-(dimethyl(phenyl)silyl)propanoate was used as the substrate. It can be used to 2.5-fold activate your downstream gene of interest as it is shown by our experiments. This part was ordered from IDT as oligo and oligo annealed, resulting in sticky ends to be golden gate assembled in pSB1C3 containing NLuc (Promega) as a reporter system. As riboswitches are only specific for one substrate this part is only validated for the use of the appropriate substrate.
-<!-- Add more about the biology of this part here
+<!-- Add more about the biology of this part here>
 ===Usage and Biology===
-It can be used to 2.5-fold activate your downstream gene of interest as it is shown by our experiments. This part was ordered from IDT as oligo and oligo annealed, resulting in sticky ends to be golden gate assembled in pSB1C3 containing NLuc (Promega) as a reporter system. As riboswitches are only specific for one substrate this part is only validated for the use of the appropriate substrate.
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