Difference between revisions of "Part:BBa K2350022:Design"

(→‎Design Notes)
 
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===Source===
 
===Source===
  
−
pPIGBACK 5'3'NSII and PrbcL are from Synechococcus elongatus PCC7942. pPIGBACK ori and ampicillin resistant gene are from pBR322. CrtZ is from Pantoea ananatis.
+
pPIGBACK is from BBa_K2350009, and CrtZ is from Pantoea ananatis.
  
 
===References===
 
===References===

Latest revision as of 13:37, 26 October 2017


pPIGBACK-PrbcL-CrtZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used fusion PCR to fuse CrtZ and PrbcL to avoid restriction site in between which might influence expression efficiency.

Source

pPIGBACK is from BBa_K2350009, and CrtZ is from Pantoea ananatis.

References