Difference between revisions of "Part:BBa K2285015"

Revision as of 13:35, 26 October 2017

J23119+Target3+amilCP(RBS+amilCP+Term)

constitutive promoter J23119 followed by a stem-loop structure Target3, with chromoprotein amilCP as the reporter gene. The original part of amilCP chromoprotein is presented on BBa_K1357009, which is a composite part with B0034 and B0010+B0012 double-terminator.

Usage and Biology

The coding region of amilGFP was originated from iGEM14_Virginia.

More infomation and details about usage of this part can refer to J23119+Target3+eforRed BBa_K2285012

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2285015 short</partinfo>
-constitutive promoter J23119 followed by a stem-loop structure Target3, with chromoprotein amilCP(BBa_K1357009) as the reporter gene. The information of part amilCP was given at the original part page, consisting of an RBS, the blue chromoprotein amilCP, and a double terminator B0010+B0012.
+constitutive promoter J23119 followed by a stem-loop structure Target3, with chromoprotein amilCP as the reporter gene. The original part of amilCP chromoprotein is presented on <partinfo>BBa_K1357009</partinfo>, which is a composite part with B0034 and B0010+B0012 double-terminator.
 ===Usage and Biology===
-During experiments, we found that expression of chromoproteins amilCP, amilGFP, eforRed and cjBlue took nearly 24 hours to see the colors with naked eyes on the LB plate. But they are still great reporters when it comes to build visualized systems which do not need to depend on extra equippments.
+The coding region of amilGFP was originated from iGEM14_Virginia.
+More infomation and details about usage of this part can refer to '''J23119+Target3+eforRed''' <partinfo>BBa_K2285012</partinfo>
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