Difference between revisions of "Part:BBa K2285014"

Latest revision as of 13:30, 26 October 2017

J23119+Target3+cjBlue(with RBS and Term added)

constitutive promoter J23119 followed by a stem-loop structure Target3, with chromoprotein cjBlue as the reporter gene. The original part of cjBlue chromoprotein is presented on BBa_K592011. What should be noticed is, the original part is a coding part and doesn't have its own RBS and Term. Therefore, to achieve greater convenience fo our Star-chromoprotein reporter system, we add the RBS which is on the upstream of sfGFP(BBa_K515005) as well as a short terminator B1006 to all chromoproteins we used in this project via PCR.

Usage and Biology

The coding region of cjBlue was originated from iGEM11_Uppsala-Sweden.

More infomation and details about usage of this part can refer to J23119+Target3+eforRed BBa_K2285012

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2285014 short</partinfo>
-constitutive promoter J23119 followed by a stem-loop structure Target3, with chromoprotein cjBlue as the reporter gene.
+constitutive promoter J23119 followed by a stem-loop structure Target3, with chromoprotein cjBlue as the reporter gene. The original part of cjBlue chromoprotein is presented on <partinfo>BBa_K592011</partinfo>. What should be noticed is, the original part is a coding part and doesn't have its own RBS and Term. Therefore, to achieve greater convenience fo our Star-chromoprotein reporter system, we add the RBS which is on the upstream of sfGFP(<partinfo>BBa_K515005</partinfo>) as well as a short terminator B1006 to all chromoproteins we used in this project via PCR.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The coding region of cjBlue was originated from iGEM11_Uppsala-Sweden.
+More infomation and details about usage of this part can refer to '''J23119+Target3+eforRed''' <partinfo>BBa_K2285012</partinfo>
 <!-- -->