Difference between revisions of "Part:BBa K2285013"

Revision as of 13:16, 26 October 2017

J23119+Target3+amilGFP(with RBS and Term added)

constitutive promoter J23119 followed by a stem-loop structure Target3, with chromoprotein amilGFP as the reporter gene. The original part of amilGFP chromoprotein is presented on BBa_K592010. What should be noticed is, the original part is a coding part and doesn't have its own RBS and Term. Therefore, to achieve greater convenience fo our Star-chromoprotein reporter system, we add the RBS which is on the upstream of sfGFP(BBa_K515005) as well as a short terminator B1006 to all chromoproteins we used in this project via PCR.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2285013 short</partinfo>
-constitutive promoter J23119 followed by a stem-loop structure Target3, with chromoprotein amilGFP as the reporter gene.
+constitutive promoter J23119 followed by a stem-loop structure Target3, with chromoprotein amilGFP as the reporter gene. The original part of amilGFP chromoprotein is presented on <partinfo>BBa_K592010</partinfo>. What should be noticed is, the original part is a coding part and doesn't have its own RBS and Term. Therefore, to achieve greater convenience fo our Star-chromoprotein reporter system, we add the RBS which is on the upstream of sfGFP(<partinfo>BBa_K515005</partinfo>) as well as a short terminator B1006 to all chromoproteins we used in this project via PCR.
 <!-- Add more about the biology of this part here