Difference between revisions of "Part:BBa I746101"

Latest revision as of 07:45, 23 October 2007

I746100 (AIP sensor infrastructure - agrC + agrA with RBSes) + B0015 (Terminator)

This part is simply the AIP sensor infrastructure (I746100) from the agr system with a standard registry terminator (B0015) downstream.

The original part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism.

In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP.

This part contains the coding regions for group I agrC and agrA (in that order) from S. aureus strain NCTC8325, prefixed by the B0034 RBS and separated by a BamHI site.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 383
Illegal BamHI site found at 1267
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_I746101 short</partinfo>
-This part is simply the AIP sensor infrastructure ([https://parts.igem.org/wiki/index.php/Part:BBa_I746001 I746001]) from the <i>agr</i> system with a standard registry terminator ([https://parts.igem.org/wiki/index.php/Part:BBa_B0015 B0015]) downstream.
+This part is simply the AIP sensor infrastructure ([https://parts.igem.org/wiki/index.php/Part:BBa_I746100 I746100]) from the <i>agr</i> system with a standard registry terminator ([https://parts.igem.org/wiki/index.php/Part:BBa_B0015 B0015]) downstream.
 The original part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism.