Difference between revisions of "Part:BBa I746008"

Revision as of 07:07, 23 October 2007

I0500 (Inducible pBad/araC) + I746001 (AIP generator - agrB + agrD with RBSes + Terminator)

This part is the AIP generator from the agr system with an inducible promoter upstream (arabinose).

pBad is an E. coli promoter that is tightly controlled by:

inducer: L-[http://openwetware.org/wiki/Arabinose arabinose].
repressor: AraC apparently acts as the repressor

The original (I746000) part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism. It contains the coding regions for group I agrB and agrD (in that order) from S. aureus strain NCTC8325, prefixed by the B0034 RBS and separated by a SaII site.

In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_I746008 short</partinfo>
@@ Line 5: / Line 4: @@
 This part is the AIP generator from the agr system with an inducible promoter upstream (arabinose).
-pBad is an E. coli promoter that is tightly controlled by:
+pBad is an ''E. coli'' promoter that is tightly controlled by:
+* inducer: L-[http://openwetware.org/wiki/Arabinose arabinose].
-   * inducer: L-arabinose.
+* repressor: AraC apparently acts as the repressor
-   * repressor: AraC apparently acts as the repressor
 The original (I746000) part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism.  It contains the coding regions for group I agrB and agrD (in that order) from S. aureus strain NCTC8325, prefixed by the B0034 RBS and separated by a SaII site.