Revision as of 11:00, 26 October 2017

Introduction

BCP+T1R3 overlap

This part is the overlap of that sweetness receptor T1R3 combines with the bcp tag.

The T1R3 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R3 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R3 and bcp tag.

The result of this part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R3.

Design

Firstly, we constructed the specific primers that consists of the overlap regions of the T1R3 and bcp. Then, we combined the overlap of T1R3 with the bcp by PCR.

Fig.1 The schematic diagram of bcp+T1R3 overlap

Fig. 2 The PCR method of bcp+T1R3 overlap

Experiment

This part is used to detect the expression and the location of the T1R3.

Fig.3 Electrophoresis of bcp+T1R3 overlap.Line two is the target.

Sequence and Features