Difference between revisions of "Part:BBa K2247000:Design"

 
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===Design Notes===
 
===Design Notes===
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Upstream activation sequence (UAS) modified to enhance the strength of the promoter
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This part is a Gal promoter from the yeast S. cerevisiae, modified from the endogenous Gal1 promoter. Endogenous Gal1 promoter contains upstream activating sequence (UAS), where lies the binding sites of the yeast Gal4 activator. Similar to pGal1, the expression from pGalS can be induced by Gal4 protein, with a higher strength than pGal1.
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===Source===
 
===Source===

Latest revision as of 07:10, 26 October 2017


pGalS promoter of S. cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 453
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a Gal promoter from the yeast S. cerevisiae, modified from the endogenous Gal1 promoter. Endogenous Gal1 promoter contains upstream activating sequence (UAS), where lies the binding sites of the yeast Gal4 activator. Similar to pGal1, the expression from pGalS can be induced by Gal4 protein, with a higher strength than pGal1.

Source

Modified by Dr. Junbiao Dai's Group from Tsinghua University

References