Difference between revisions of "Part:BBa K2505000"
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This gene is derived from <i>A.thaliana</i> and optimized for <i>E.coli</i> codon in reference to the codon usage. AHK4 is a receptor of iP(isopentenyl adenine: kind of cytokinin). Once iP received by AHK4, AHK4 induces histidine kinase phosphorylation in <i>E.coli</i> cell. Then, arbitrary promoter is activated by the phosphorylation.
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This gene is derived from <i>A.thaliana</i> and optimized for <i>E.coli</i> codon in reference to the codon usage. The protein(AHK4)  is a receptor of iP(isopentenyl adenine: kind of cytokinin). Once iP received by AHK4, AHK4 induces histidine kinase phosphorylation in <i>E.coli</i> cell. Then, arbitrary promoter is activated by the phosphorylation.
  
  

Revision as of 04:48, 26 October 2017

rbs-ahk4

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 76
    Illegal BamHI site found at 1157
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 324
    Illegal SapI.rc site found at 1959


This gene is derived from A.thaliana and optimized for E.coli codon in reference to the codon usage. The protein(AHK4) is a receptor of iP(isopentenyl adenine: kind of cytokinin). Once iP received by AHK4, AHK4 induces histidine kinase phosphorylation in E.coli cell. Then, arbitrary promoter is activated by the phosphorylation.


Characterization

We tested the function of AHK4 protein that receives iP and induces histidine kinase phosphorylation in E.coli cell. Our objective was to characterize the function of AHK4 protein.

Materials & methods

Result