Difference between revisions of "Part:BBa K2407104:Design"

 
 
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
 
This sequence is modified for use in yeast or other AT-rich fungi.
 
This sequence is modified for use in yeast or other AT-rich fungi.
−
 
+
This part is a Modified-Gal inducable promoter working with the TF Z4EV.
−
 
+
As a well functioned terminator, this part can perform well to control the expression of HO gene.
  
 
===Source===
 
===Source===
  
−
obtain from Gene synthesis company after codon optimization
+
It is obtained from Gene synthesis company after codon optimization
  
  
 
===References===
 
===References===
 +
Houseley J1, Rubbi L, Grunstein M, Tollervey D, Vogelauer M.  A ncRNA modulates histone modification and mRNA induction in the yeast GAL gene cluster.10.1016/j.molcel.2008.09.027.

Latest revision as of 04:38, 26 October 2017


Modified-Gal inducable promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 315
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is modified for use in yeast or other AT-rich fungi. This part is a Modified-Gal inducable promoter working with the TF Z4EV. As a well functioned terminator, this part can perform well to control the expression of HO gene.

Source

It is obtained from Gene synthesis company after codon optimization


References

Houseley J1, Rubbi L, Grunstein M, Tollervey D, Vogelauer M. A ncRNA modulates histone modification and mRNA induction in the yeast GAL gene cluster.10.1016/j.molcel.2008.09.027.